Base composition and metabolism of various RNA fractions in Acetabularia mediterranea.
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چکیده
منابع مشابه
Base composition and metabolism of various RNA fractions in Acetabularia mediterranea.
The fact that anucleate fragments of Acetabularia mediterranea are capable of regeneration and morphogenesis and of specific protein synthesis has been known for many years." 2 The information needed for these processes obviously comes from the nucleus and must be stored for a considerable time in the cytoplasm: this can be most readily explained in molecular terms by assuming that the nucleus ...
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The cytoplasm of an Acetabularia cell is normally at a potential of about -170 mv relative to the external solution; the vacuole is also at this potential. Although there is strict flux equilibrium for all ions, the potential is more negative than the Nernst potentials of any of the permeating ions. Darkness, CCCP, low temperature, and reducing [Cl(-)](o) by a factor of 25 all rapidly depolariz...
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Radioautographic and radiochemical techniques were used to establish the presence of replicating DNA in the chloroplasts of Acetabularia mediterranea. These techniques also demonstrated that these chloroplasts synthesize RNA. It was found that label from thymine was also incorporated into DNA and RNA in these chloroplasts. With the establishment of protein and nucleic acid synthesis in Acetabul...
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The structural organization of transcriptionally active DNA that contains cistrons for precursor molecules of ribosomal RNA is described in positively stained spread preparations from nuclei and nucleoli isolated from the green alga, Acetabularia mediterranea Lmx. These nuclei contain large aggregates of nucleolar subunits in which fibril-covered regions, the putative active cistrons for precur...
متن کاملBase composition of RNA fractions of nuclei of Walker tumor isolated with the citric acid procedure.
RNA was extracted from tumor nuclei isolated with the citric acid procedure. The RNA was subfractionated with phenol, and fractions of differing sedimentation constants were separated on sucrose density gradients. Each peak was refractionated by repeated sucrose density gradient centrifugaron until a sym metric peak was obtained. After phenol fractionation, the interphase or iRNA obtained by ex...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1968
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.59.2.406